Evidence for RNA-Oligonucleotides in Plant Vacuoles Isolated from Cultured Tomato Cells

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Evidence for RNA-Oligonucleotides in Plant Vacuoles Isolated from Cultured Tomato Cells.

We have shown that highly purified vacuoles of suspension-cultured tomato (Lycopersicon esculentum) cells contain RNA-oligonucleotides, using two different approaches to label and detect RNA: (a) in vivo labeling of cellular RNA with [5-(3)H]uridine, followed by preparation of vacuoles from protoplasts and by quantification of radioactively labeled material; and (b) in vitro labeling and analys...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1990

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.94.3.1163